Osteogenic Effect of Fisetin Doping in Bioactive Glass/Poly(caprolactone) Hybrid Scaffolds - Université Clermont Auvergne Access content directly
Journal Articles ACS Omega Year : 2022

Osteogenic Effect of Fisetin Doping in Bioactive Glass/Poly(caprolactone) Hybrid Scaffolds

Abstract

Treating large bone defects or fragile patients may require enhancing the bone regeneration rate to overcome a weak contribution from the body. This work investigates the osteogenic potential of nutrient fisetin, a flavonoid found in fruits and vegetables, as a doping agent inside the structure of a SiO 2 − CaO bioactive glass−poly(caprolactone) (BG−PCL) hybrid scaffold. Embedded in the full mass of the BG− PCL hybrid during one-pot synthesis, we demonstrate fisetin to be delivered sustainably; the release follows a first-order kinetics with active fisetin concentration being delivered for more than 1 month (36 days). The biological effect of BG−PCL−fisetin-doped scaffolds (BG−PCL−Fis) has been highlighted by in vitro and in vivo studies. A positive impact is demonstrated on the adhesion and the differentiation of rat primary osteoblasts, without an adverse cytotoxic effect. Implantation in critical-size mouse calvaria defects shows bone remodeling characteristics and remarkable enhancement of bone regeneration for fisetin-doped scaffolds, with the regenerated bone volume being twofold that of nondoped scaffolds and fourfold that of a commercial trabecular bovine bone substitute. Such highly bioactive materials could stand as competitive alternative strategies involving biomaterials loaded with growth factors, the use of the latter being the subject of growing concerns.
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Dates and versions

hal-03857797 , version 1 (17-11-2022)

Licence

Attribution - CC BY 4.0

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Cite

Henri Granel, Cédric Bossard, Anne-Margaux Collignon, Fabien Wauquier, Julie Lesieur, et al.. Osteogenic Effect of Fisetin Doping in Bioactive Glass/Poly(caprolactone) Hybrid Scaffolds. ACS Omega, 2022, 7 (26), pp.22279-22290. ⟨10.1021/acsomega.2c01109⟩. ⟨hal-03857797⟩
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