HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Lysine and homoarginine are closely interrelated metabolites in the rat

Abstract : L-Lysine (Lys) and L-arginine (Arg), but not L-homoarginine (hArg), are proteinogenic amino acids. In healthy humans, oral administration of hArg increased the plasma concentration of Lys, suggesting Lys as a metabolite of hArg. In humans and animals, hArg is biosynthesized from Arg and Lys by arginine:glycine amidinotransferase (AGAT). In vitro, recombinant human arginase and bovine liver arginase I hydrolyzed hArg to Lys, suggesting Lys as a metabolite of hArg. The aim of the present study was to investigate whether changes in blood concentrations of hArg and Lys in old rats fed for 4 months with varied controlled experimental diets could suggest interconversion of these amino acids. Blood samples (n = 253) were taken before (T0) and after 2 months (T2) and 4 months (T4) of the experiment. Plasma concentrations of Lys and hArg were determined by gas chromatography-mass spectrometry. The plasma hArg concentration markedly correlated with the plasma Lys concentration at all timepoints (r ≥ 0.7, P < 0.0001). Further analysis demonstrated that hArg and Lys are closely and specifically associated independently of experimental time/rat age and diet, suggesting that hArg and Lys are mutual metabolites in old rats. Based on the plasma concentration changes, the median yield of hArg from Lys was determined to be 0.17% at T0 and each 0.27% at T2 and T4. With a circulating concentration of about 3 µM, hArg a major metabolite of Lys in healthy humans. hArg supplementation is currently investigated as a cardioprotective means to improve impaired hArg synthesis. Present knowledge suggests that Lys rather than hArg supplementation may be even more favorable.
Complete list of metadata

https://hal.uca.fr/hal-03655502
Contributor : Laurent Mosoni Connect in order to contact the contributor
Submitted on : Thursday, May 5, 2022 - 9:00:37 AM
Last modification on : Tuesday, May 10, 2022 - 1:57:15 PM

File

Baskal2022_Article_LysineAndHo...
Publication funded by an institution

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Citation

Svetlana Baskal, Laurianne Dimina, Stefanos A. Tsikas, Laurent Mosoni, Didier Remond, et al.. Lysine and homoarginine are closely interrelated metabolites in the rat. Amino Acids, Springer Verlag, 2022, ⟨10.1007/s00726-022-03158-0⟩. ⟨hal-03655502⟩

Share

Metrics

Record views

0

Files downloads

0