Skip to Main content Skip to Navigation
Journal articles

A combined LC-MS and NMR approach to reveal metabolic changes in the hemolymph of honeybees infected by the gut parasite Nosema ceranae

Abstract : Nosema ceranae is an emerging and invasive gut pathogen in Apis mellifera and is considered as a factor contributing to the decline of honeybee populations. Here, we used a combined LC-MS and NMR approach to reveal the metabolomics changes in the hemolymph of honeybees infected by this obligate intracellular parasite. For metabolic profiling, hemolymph samples were collected from both uninfected and N. ceranae-infected bees at two time points, 2 days and 10 days after the experimental infection of emergent bees. Hemolymph samples were individually analyzed by LC-MS, whereas each NMR spectrum was obtained from a pool of three hemolymphs. Multivariate statistical PLS-DA models clearly showed that the age of bees was the parameter with the strongest effect on the metabolite profiles. Interestingly, a total of 15 biomarkers were accurately identified and were assigned as candidate biomarkers representative of infection alone or combined effect of age and infection. These biomarkers included carbohydrates (α/β glucose, α/β fructose and hexosamine), amino acids (histidine and proline), dipeptides (Glu-Thr, Cys-Cys and γ-Glu-Leu/Ile), metabolites involved in lipid metabolism (choline, glycerophosphocholine and O-phosphorylethanolamine) and a polyamine compound (spermidine). Our study demonstrated that this untargeted metabolomics-based approach may be useful for a better understanding of pathophysiological mechanisms of the honeybee infection by N. ceranae.
Complete list of metadata

https://hal.uca.fr/hal-03132817
Contributor : Stéphanie Bonnefoy <>
Submitted on : Friday, February 5, 2021 - 2:03:00 PM
Last modification on : Friday, April 9, 2021 - 3:33:20 AM

Identifiers

Citation

Cyril Jousse, Céline Dalle, Angélique Abila, Mounir Traïkia, Marie Diogon, et al.. A combined LC-MS and NMR approach to reveal metabolic changes in the hemolymph of honeybees infected by the gut parasite Nosema ceranae. Journal of Invertebrate Pathology, Elsevier, 2020, 176, pp.107478. ⟨10.1016/j.jip.2020.107478⟩. ⟨hal-03132817⟩

Share

Metrics

Record views

48