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Hydrolysis and fermentation steps of a pretreated sawmill mixed feedstock for bioethanol production in a wood biorefinery

Abstract : the aim of this work was to demonstrate the feasibility of second-generation bioethanol production using for the first time a sawmill mixed feedstock comprising four softwood species, representative of biomass resource in auvergne-rhone-alpes region (france). the feedstock was subjected to a microwave-assisted water/ethanol organosolv pretreatment. the investigation focused on enzymatic hydrolysis of this pretreated sawmill feedstock (psf) using cellic® ctec2 as the enzyme, followed by fermentation of the resulting sugar solution using saccharomyces cerevisiae strain. the cellulose-rich psf with 71% w/w cellulose content presented high saccharification yields (up to 80%), which made it perfect for subsequent fermentation; this yield was predicted vs. time up to 5.2% w/v psf loading using a mathematical model fitted only on data at 1.5%. finally, high psf loading (7.5%) and scaleup were shown to impair the saccharification yield, but alcoholic fermentation could still be carried out up to 80% of the theoretical glucose-to-ethanol conversion yield.
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https://hal.uca.fr/hal-03086115
Contributor : Christophe Tournayre Connect in order to contact the contributor
Submitted on : Tuesday, December 22, 2020 - 12:08:00 PM
Last modification on : Wednesday, February 24, 2021 - 4:16:03 PM

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Maarouf Abdou-Alio, O Tugui, L Rusu, Agnès Pons, Christophe Vial. Hydrolysis and fermentation steps of a pretreated sawmill mixed feedstock for bioethanol production in a wood biorefinery. Bioresource Technology, Elsevier, 2020, 310, pp.123412. ⟨10.1016/j.biortech.2020.123412⟩. ⟨hal-03086115⟩

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