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Centrin-like filaments in the cytopharyngeal apparatus of the ciliatesNassula andFurgasonia: Evidence for a relationship with microtubular structures

Abstract : The cytopharyngeal apparatus in the Nassulinid ciliates Nassula and Furgasonia is a highly specialized microtubular/filamentous organelle designed for ingestion of organisms such as filamentous bacteria. From studies on living cells, it was previously shown that this organelle, also called "feeding basket," guides the filamentous bacteria and manipulates them to some extent during the early steps of ingestion. This results in a complex sequence of movements where the basket is successively dilated and constricted in its upper part. Whereas some of these movements (dilation) seem to be intrinsic to the microtubular components of the basket, others (constriction) are believed to be mediated by contractile filamentous structures [Tucker, 1968: J. Cell Sci. 3:493-514]. In this study, we have used antibodies raised against ciliate centrins to demonstrate these proteins by Western blot and immunocytochemical methods in Nassula and Furgasonia. In both ciliates, a 20-kDa centrin immunoanalog was localized in the upper (contractile) part of the cytopharyngeal apparatus. Immunoelectron microscopy revealed that cytopharyngeal centrin is engaged in filamentous material, forming a sphincter-like structure possibly involved in the movements of contraction. Interestingly, physical links were noted between filaments labeled for centrin and cytopharyngeal microtubules. The mechanistic implications of these findings are discussed.
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https://hal.uca.fr/hal-02866751
Contributor : Philippe Bouchard <>
Submitted on : Friday, June 12, 2020 - 5:14:53 PM
Last modification on : Saturday, June 13, 2020 - 3:37:53 AM

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  • HAL Id : hal-02866751, version 1
  • PUBMED : 10340705

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Bernard Vigues, Marie-Pierre Blanchard, Philippe Bouchard. Centrin-like filaments in the cytopharyngeal apparatus of the ciliatesNassula andFurgasonia: Evidence for a relationship with microtubular structures. Cell Motility and the Cytoskeleton, Wiley-Blackwell, 1999, 43 (1), pp.72-81. ⟨hal-02866751⟩

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