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Improved protocol for high-quality Co-extraction of DNA and RNA from rumen digesta

Abstract : We report an improved method for total nucleic acids extraction from rumen content samples. The method employs bead beating, and phenol–chloroform extraction followed by saline–alcohol precipitation. Total nucleic acids and RNA yield and purity were assessed by spectrophotometric measurements; RNA integrity was estimated using Agilent RNA 6000 Nano Kit on an Agilent 2100 Bioanalyzer. The method provided total nucleic acids and RNA extracts of good quantity and quality. The extraction is not time consuming and it is valuable for ecological studies of rumen microbial community structure and gene expression.
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https://hal.uca.fr/hal-02454699
Contributor : Milka Popova <>
Submitted on : Friday, January 24, 2020 - 4:59:02 PM
Last modification on : Saturday, May 30, 2020 - 7:28:33 PM

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Milka Popova, C. Martin, D. Morgavi. Improved protocol for high-quality Co-extraction of DNA and RNA from rumen digesta. Folia Microbiologica, Springer Verlag (Germany), 2010, 55 (4), pp.368-372. ⟨10.1007/s12223-010-0060-3⟩. ⟨hal-02454699⟩

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