Skip to Main content Skip to Navigation
Journal articles

Curative Treatment of Candidiasis by the Live Biotherapeutic Microorganism Lactobacillus rhamnosus Lcr35 in the Invertebrate Model Caenorhabditis elegans: First Mechanistic Insights

Abstract : The resistance of Candida albicans to conventional drug treatments, as well as the recurrence phenomena due to dysbiosis caused by antifungal treatments, have highlighted the need to implement new therapeutic methodologies. The antifungal potential of live biotherapeutic products (LBP) has already been demonstrated using preclinical models (cell cultures, laboratory animals). Understanding their mechanisms of action is strategic for the development of new therapeutics for humans. In this study, we investigated the curative anti-C. albicans properties of Lactobacillus rhamnosus Lcr35 ® using the in vitro Caco-2 cell and the in vivo Caenorhabditis elegans models. We showed that Lcr35 ® does inhibit neither the growth (p = 0.603) nor the biofilm formation (p = 0.869) of C. albicans in vitro. Lcr35 ® protects the animal from the fungal infection (+225% of survival, p < 2 × 10-16) even if the yeast is detectable in its intestine. In contrast, the Lcr35 ® cell-free supernatant does not appear to have any antipathogenic effect. At the mechanistic level, the DAF-16/Forkhead Box O transcription factor is activated by Lcr35 ® and genes of the p38 MAP Kinase signaling pathway and genes involved in the antifungal response are upregulated in presence of Lcr35 ® after C. albicans infection. These results suggest that the LBM strain acts by stimulating its host via DAF-16 and the p38 MAPK pathway.
Complete list of metadatas

Cited literature [50 references]  Display  Hide  Download

https://hal.uca.fr/hal-02432425
Contributor : Cyril Poupet <>
Submitted on : Wednesday, January 8, 2020 - 2:17:40 PM
Last modification on : Tuesday, March 17, 2020 - 2:31:14 AM

File

microorganisms-08-00034.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Cyril Poupet, Philippe Veisseire, Muriel Bonnet, Olivier Camares, Marylise Gachinat, et al.. Curative Treatment of Candidiasis by the Live Biotherapeutic Microorganism Lactobacillus rhamnosus Lcr35 in the Invertebrate Model Caenorhabditis elegans: First Mechanistic Insights. Microorganisms, MDPI, 2019, ⟨10.3390/microorganisms8010034⟩. ⟨hal-02432425⟩

Share

Metrics

Record views

106

Files downloads

139