Constraining arc magma sources at continental arc settings is a delicate task, because chemical signatures from crustal processes obscure the slab and mantle signatures. Here, we present major, trace, and volatile element compositions of olivine-hosted melt inclusions (Fo82–89) selected from the most primitive lavas (Mg# > 60) from two Ecuadorian volcanoes (Puñalica and Sangay) situated at the southern termination of the Andean Northern Volcanic Zone. Melt inclusions (MI) from Puñalica are nepheline normative and have basaltic-to-basaltic-andesite compositions (45–56 wt% SiO2) similar to peridotite-derived melts. Sangay MI is also nepheline normative, with high CaO (up to 16 wt% and CaO/Al2O3 < 1) and low silica contents (41.9–44.5 wt%) pointing out an amphibole-bearing clinopyroxenite source. Both volcanoes display volatile-rich compositions (up to 6100 ppm Cl, 2200 ppm F, and 6700 ppm S). These MI cannot be related to their host lavas by fractional crystallization, implying that they represent true primitive liquids. The source of Puñalica MI was metasomatized by slab-derived melts that imprints its low Ba/Th, Sr/Th, and high Th/La (average values of 66, 129, and 0.22, respectively). On the contrary, the slab component added to the source of Sangay MI has a higher Ba/Th, Sr/Th, and low Th/La (average values of 261, 517, and 0.11, respectively) which could suggest a relative contribution of aqueous fluids. This dichotomy is related to the presence of the Grijalva Fracture Zone that separates a younger and hotter oceanic crust to the north (below Puñalica) from a colder and older oceanic crust to the south (below Sangay).