Quantification of 4-hydroxy-2-nonenal-protein adducts in in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation - Université Clermont Auvergne Accéder directement au contenu
Article Dans Une Revue Food and Function Année : 2016

Quantification of 4-hydroxy-2-nonenal-protein adducts in in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation

Mylène Delosière
Charlotte Joly
Estelle Pujos-Guillot
Didier Remond
Blandine Comte
Cécile Gladine

Résumé

Hydroxyalkenals are lipid oxidation end-products resulting from the oxidation of polyunsaturated fatty acids (PUFA). This study aimed at quantifying the production of 4-hydroxy-2-nonenal-protein adducts (HNE-P) via Michael addition from n-6 PUFA oxidation in the gastric digesta of mini-pigs after the consumption of meat-based meals with different plant antioxidant contents. Using the accuracy profile procedure, we validated an extraction protocol for the quantification of HNE-P by GC-MS/MS in gastric contents. The formation of HNE-P in the gastric compartment was observed for the first time, with concentrations ranging from less than 0.52 to 1.33 nmol HNE-P / 500 mg digesta. Nevertheless, most gastric HNE-P levels were below the limit of quantification of 0.52 nmol HNE-P / 500 mg digesta. In this animal study, the protective effect of plant antioxidant sources on HNE-P formation was not evidenced contrasting with results using TBARS as markers.
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Dates et versions

hal-01901450 , version 1 (22-10-2018)

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Citer

Mylène Delosière, Véronique Santé-Lhoutellier, Céline Chantelauze, Denys Durand, Agnes Thomas, et al.. Quantification of 4-hydroxy-2-nonenal-protein adducts in in vivo gastric digesta of mini-pigs using a GC-MS/MS method with accuracy profile validation. Food and Function, 2016, 7 (8), pp.3497-3504. ⟨10.1039/C6FO00413J⟩. ⟨hal-01901450⟩
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