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Poster De Conférence Année : 2012

Gadolinium based nanoparticles as radiosensitizing agents for radioresistant head and neck tumours

Résumé

Radiotherapy is one of the main therapies for cancer, but it remains limited when tumours are radioresistant such glioblastoma, head and neck tumours, osteo and chondrosarcomas for example. Several strategies aiming at radiosensitizing these resistant tumours are under development. One of those relies on the use of high density nanoparticles, which once delivered into the tumour should deliver secondary radiations amplifying the radiotherapy efficiency. A previous reported study from our consortium showed that Gadolinium Based Nanoparticles (GBN) were able to induce a clonogenic death of SQ20B cells in culture (human head and neck carcinoma cells). The present study aimed at the demonstration of the in-vivo radiosensitizing effect of these GBN on SQ20B xenograft tumour. Radiation Therapy (RT) was performed using a biological X-Ray Irradiator (X-RAD 320) (Byfleet, Surrey) with 320 KV X-rays. The field of irradiation was adapted according to tumor volume by using an adjustable collimator positioned 35 cm from the animals. The dose rate was of 2 Gy.min-1. Four/five weeks after tumor grafting, mice with tumor volume less than 400 mm3 were randomly selected for radiotherapy 5 minutes after intratumoral (IT) injection of either GBN or saline. Four groups of rats were compared: IT injection of saline only (Control) (n = 9), GBN IT injection only (GBN injection) (n = 9), IT injection of saline + 10 Gy radiation (IR 10Gy) (n = 13), and IT injection of GBN + 10 Gy (IR 10Gy + GBN) (n = 10). Compared to the progressive and massive increase in tumor volume observed in the control groups (control and GBN injection groups), 10 Gy radiation stabilized tumor evolution between the second and the third week after radiation. The tumors then started to develop again with a growth curve similar to that of the control group. In contrast, the combination of GBN with 10 Gy radiation limited spectacularly the SQ20B tumors growth. At the end of week 6, the mean reduction in tumor volume in the IR 10Gy + GBN group, was of 626 and 388% (P = 0,003) compared, respectively, to the control and only irradiated tumors. Moreover, in the end of our therapy study, tumors have disappeared in two mice given 10 Gy with GBN treatment. Combined with the in-vitro study, the reported results demonstrate a major radiosensitizing effect of Gadolinium Based Nanoparticles on radioresistant tumours such as Head and Neck carcinomas.
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Dates et versions

hal-00838604 , version 1 (26-06-2013)

Identifiants

  • HAL Id : hal-00838604 , version 1

Citer

I. Miladi, M.-T. Aloy, A. Berniard, E. Armandy, P. Mowat, et al.. Gadolinium based nanoparticles as radiosensitizing agents for radioresistant head and neck tumours. World Molecular Imaging Congress, Sep 2012, Dublin, Ireland. ⟨hal-00838604⟩
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